Ozone Dialysis (EBO2 Therapy) for Anti-Aging and Cellular Health

As the global population ages, more people than ever are living well beyond 50. Along with longer life comes a rise in age-related conditions such as heart disease, diabetes, arthritis, and neurodegenerative disorders.

At the heart of this health challenge is inflammaging, a slow, chronic, low-grade inflammation that accumulates over time. Imagine a small, persistent fire inside the body. It doesn’t hurt immediately, but over years, this inflammation damages cells, accelerates aging, and contributes to nearly every chronic illness.

Several interconnected biological factors drive chronic inflammation:

1. Oxidative Stress – Aging cells produce more reactive oxygen species (ROS) while antioxidant defenses weaken, damaging DNA, proteins, and lipids.
2. DAMPs (Damage-Associated Molecular Patterns) – Injured or stressed cells release DAMPs, signaling inflammation even without infection.
3. Cellular Senescence – Aging cells may enter a “zombie” state called senescence, stopping division but continuing to release harmful inflammatory compounds.
4. SASP (Senescence-Associated Secretory Phenotype) – Senescent cells secrete inflammatory cytokines and enzymes, spreading inflammation to nearby tissues.
5. Immunosenescence – Aging immune cells become less effective at fighting infections and more prone to releasing inflammatory signals, amplifying inflammaging.

The body has an internal mechanism to combat oxidative stress and inflammation: the Nrf2-HO-1 pathway. Think of Nrf2 (Nuclear factor erythroid 2-related factor 2) as a master switch for cellular defense. When activated, it triggers protective genes, including heme oxygenase-1 (HO-1), glutathione, and superoxide dismutase (SOD).

HO-1 protects cells by breaking down heme, a pro-oxidant, into:

• Biliverdin/Bilirubin – potent antioxidants
• Carbon Monoxide (CO) – anti-inflammatory signaling molecule in small amounts
• Iron/Ferritin – safely stores iron, preventing oxidative damage

High HO-1 levels help cells resist oxidative stress, inflammation, and tissue damage, all critical for slowing aging and reducing chronic disease risk.

As we age, Nrf2 activity naturally decreases, creating a cycle of inflammation and oxidative stress, known as the Oxi-Inflamm-Aging Loop:

• Oxidative stress triggers inflammation
• Inflammation generates more ROS
• ROS causes further cellular damage

This cycle accelerates cellular aging and contributes to many chronic illnesses.

Ozone Dialysis, also referred to as EBO2 Therapy (Extracorporeal Blood Oxygenation and Ozonation), is an advanced regenerative therapy that:

• Withdraws a small portion of your blood
• Exposes it to a precise mixture of oxygen and ozone
• Returns the treated blood to your body

While the procedure is simple, its effects on cellular health are profound, stimulating the Nrf2-HO-1 pathway, enhancing antioxidant defenses, and reducing chronic inflammation.

Hormetic Stress Response – Mild oxidative stress from ozone triggers protective genes, similar to how exercise strengthens muscles.

Enhanced Nrf2 Activation – Ozone stimulates multiple pathways that keep Nrf2 active for days, prolonging antioxidant and anti-inflammatory benefits.

Increased HO-1 Production – Boosts HO-1 levels in circulating blood cells, enhancing cellular protection.

Systemic Anti-Inflammatory Effects – HO-1 byproducts such as bilirubin, CO, and ferritin reduce cytokine activity, neutralize ROS, and lower inflammation body-wide.

Slows Cellular Aging – Reduces oxidative stress and inflammation, decreases senescent cell accumulation, and supports healthier immune function.

Immune System Rejuvenation – Improves regulatory T-cell and natural killer (NK) cell activity while calming overactive inflammatory responses.

Red Blood Cell Health – Enhances RBC flexibility, oxygen delivery, and anti-inflammatory properties to support tissue repair and overall vitality.

Ozone Dialysis and EBO2 therapy have been shown to support multiple health areas:

• Heart and Vascular Health: Reduces oxidized LDL and vascular inflammation
• Autoimmune Disorders: Balances immune response without suppressing essential function
• Brain Health: Reduces neuroinflammation linked to Alzheimer’s and Parkinson’s
• Metabolic Health: Improves insulin sensitivity and lowers inflammation in fat tissues

Ozone Dialysis is emerging as a key component of personalized longevity medicine. Future advancements may include:

• Combining ozone dialysis with targeted Nrf2 activators
• Tailoring therapy based on individual inflammation and oxidative stress profiles
• Preventive protocols for those at risk of age-related disease

You can receive professional, medically supervised Ozone Dialysis at BionwoRx: Center for Functional and Regenerative Medicine, conveniently located in Carmel, Indiana, just north of Indianapolis.

We proudly serve patients from Carmel, Westfield, Fishers, Zionsville, Noblesville, and the greater Indianapolis area.